Journal: International heart journal
Article Title: Hydroxycitric Acid Tripotassium Hydrate Attenuates Monocrotaline and Hypoxia-Induced Pulmonary Hypertension in Rats.
doi: 10.1536/ihj.23-350
Figure Lengend Snippet: Figure 6. HCA inhibited the proliferation and migration of PASMCs induced by hypoxia. A: The PASMC proliferation activity detected by CCK-8. B: Representative images of PASMCs proliferation measured using EdU. C: EdU quantification. D: Representative images of the cell scratch test. E: Quantification of the cell scratch test (%, cell scratch area ratio). HCA indicates hydroxycitric acid tripotassium hydrate; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; and ns, P > 0.05.
Article Snippet: Cell proliferation assay: PASMC proliferation was examined using the Cell Counting Kit-8 (Beyotime, China) assay and the EdU Cell Proliferation Kit with Alexa Fluor 488 (Beyotime, China) assay following the manufacturer’s instructions.
Techniques: Migration, Activity Assay, CCK-8 Assay